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mouse anti gbb  (Developmental Studies Hybridoma Bank)


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    Structured Review

    Developmental Studies Hybridoma Bank mouse anti gbb
    Mouse Anti Gbb, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+gbb/anti-Glass+bottom+boat/pm39475469-352-13-17
    Average 90 stars, based on 1 article reviews
    mouse anti gbb - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: BMP-dependent synaptic development requires Abi-Abl-Rac signaling of BMP receptor macropinocytosis
    Article Snippet: For production of Gbb-conditioned medium, S2R+ cells transfected with pAc - gbb were incubated in serum-free Schneider’s medium (Gibco) for 120 h. Multiple Tag Fusion (GenScript) was used as protein standard to determine HA-Gbb concentration by western blot using rabbit anti-HA (1:1000; Cell Signaling). .. Conditioned medium with known HA-Gbb concentration was used as a standard for determining Gbb concentration by western blot using mouse anti-Gbb (GBB 3D6-24; 1:500; DSHB). .. A GST-Abi-C (amino acids 253–473) fusion protein expressed in E . coli BL21 (Stratagene) was purified with glutathione Sepharose 4B (GE Healthcare).

    Western Blot:

    Article Title: BMP-dependent synaptic development requires Abi-Abl-Rac signaling of BMP receptor macropinocytosis
    Article Snippet: For production of Gbb-conditioned medium, S2R+ cells transfected with pAc - gbb were incubated in serum-free Schneider’s medium (Gibco) for 120 h. Multiple Tag Fusion (GenScript) was used as protein standard to determine HA-Gbb concentration by western blot using rabbit anti-HA (1:1000; Cell Signaling). .. Conditioned medium with known HA-Gbb concentration was used as a standard for determining Gbb concentration by western blot using mouse anti-Gbb (GBB 3D6-24; 1:500; DSHB). .. A GST-Abi-C (amino acids 253–473) fusion protein expressed in E . coli BL21 (Stratagene) was purified with glutathione Sepharose 4B (GE Healthcare).

    Immunoprecipitation:

    Article Title: Crimpy enables discrimination of pre and postsynaptic pools of a BMP at the Drosophila NMJ
    Article Snippet: .. Immunoprecipitation Lysates were immunoprecipitated with mouse anti-Gbb (Gbb-3D6-24, DSHB). .. The membrane was probed with rat anti-HA at 1:1000 (Roche) and rabbit anti-Myc at 1:300 (Cell Signaling).

    Article Title: Crimpy enables discrimination of pre and postsynaptic pools of a BMP at the Drosophila NMJ
    Article Snippet: .. Lysates were immunoprecipitated with mouse anti-Gbb (Gbb-3D6-24, DSHB). .. The membrane was probed with rat anti-HA at 1:1000 (Roche) and rabbit anti-Myc at 1:300 (Cell Signaling).

    Incubation:

    Article Title: Postsynaptic BMP signaling regulates myonuclear properties in Drosophila larval muscles
    Article Snippet: Larvae were dissected to optimally visualize the body wall muscles in ice-cold HL3.1 buffer and fixed with 4% paraformaldehyde in HL3.1 for 20 minutes for all antibodies except GluRIIA for which samples were fixed in Bouin’s fixative (Electron Microscopy Sciences, 15990) for 5 minutes. .. Larval fillets were blocked in PBT-BSA [0.1% BSA, 0.3% Triton X-100 in PBS pH 7.4] for 30 minutes and incubated with the following primary antibodies overnight at 4 °C: mouse anti-Lamin C (DSHB, LC28.26; 1:50), mouse anti-Discs large (DSHB, 4F3; 1:200-1:300), guinea pig anti-phosphorylated Smad 1/5/8 (a gift from E. Laufer; 1:500; previously shown to be specific to Drosophila phosphorylated Mad in ), mouse anti-GluRIIA (DSHB, 8B4D2 (MH2B); 1:50), or mouse anti-Gbb (DSHB, 3D6-24; 1:50). ..



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    Image Search Results


    Journal: eLife

    Article Title: Starvation-induced regulation of carbohydrate transport at the blood–brain barrier is TGF-β-signaling dependent

    doi: 10.7554/eLife.62503

    Figure Lengend Snippet:

    Article Snippet: Antibody , anti-Gbb mouse monoclonal , Developmental Studies Hybridoma Bank , GBB 3D6-24 , (1:20).

    Techniques: Expressing, Recombinant, Plasmid Preparation, Sequencing, Cloning, Software